Enter your TB-500 vial size and the volume of bacteriostatic water you are adding. The calculator returns the concentration in mcg/ml and the exact units to draw on a U-100 insulin syringe. It works for the common 5mg and 10mg vials at loading and maintenance doses.
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Results
Concentration
5000 mcg/ml
5 mg/ml
Volume to inject
0.5 ml
per dose
Units on insulin syringe (U-100)
50 units
on a standard 100-unit insulin syringe
Doses per vial
4 doses
at 2500 mcg each
How this is calculated
10mg × 1,000 = 10000 mcg total
10000 ÷ 2ml = 5000 mcg/ml
2500 mcg ÷ 5000 = 0.5 ml
0.5 ml × 100 = 50 units
Disclaimer: This calculator is provided for informational purposes only. Always verify results against your specific vial labelling before use. Peptidy does not provide medical advice — consult a qualified healthcare professional before beginning or modifying any protocol. Use of research peptides may be subject to legal restrictions in your jurisdiction.
TB-500 (a synthetic fragment of Thymosin Beta-4) is a research peptide usually run as a loading phase of a few milligrams per week, followed by a lower maintenance dose. Because a single dose can be a large fraction of the vial, the reconstitution concentration determines whether your injection volume is comfortable.
A commonly used loading-then-maintenance structure. TB-500 is typically injected subcutaneously in the abdomen regardless of injury location, as its effects are considered systemic.
| Loading phase (weeks 1–6) | 2–2.5 mg (2,000–2,500 mcg) twice weekly |
| Maintenance | 2–2.5 mg (2,000–2,500 mcg) once every 1–2 weeks |
Worked example: 10mg vial + 2ml BAC water, 2.5mg dose
2ml is a common choice, giving 5,000 mcg/ml. A 2.5mg dose is then 0.5ml = 50 units on a U-100 insulin syringe. If 50 units feels like a lot of liquid, add less water — 1ml gives 10,000 mcg/ml and the same dose becomes 25 units.
With a 10mg vial reconstituted in 2ml (5,000 mcg/ml): 2,000 ÷ 5,000 = 0.4ml = 40 units. With a 5mg vial in 2ml (2,500 mcg/ml): 2,000 ÷ 2,500 = 0.8ml = 80 units. Enter your own vial and water volume above for an exact figure.
At a 2.5mg dose a 10mg vial provides 4 doses. During a twice-weekly loading phase that is two weeks of coverage; on a once-every-two-weeks maintenance schedule it lasts about two months. The doses-per-vial figure updates when you change the dose above.
Refrigerate at 2–8°C, keep it out of the light, and label the vial with the reconstitution date. Reconstituted TB-500 in bacteriostatic water is commonly used within 4–6 weeks. Do not freeze it.
Unlike some protocols for BPC-157, TB-500 is usually injected subcutaneously in the abdomen regardless of where an injury is, because its effects are considered systemic. The reconstitution calculation is the same wherever you inject.
Store your vial concentration, log every dose against it, and never lose track of what you injected or when.