Peptide

SS-31 Reconstitution Calculator

Enter your SS-31 (Elamipretide) vial size and the volume of bacteriostatic water you are adding. The calculator returns the concentration in mcg/ml and the exact units to draw on a U-100 insulin syringe. It works for the common 10mg and 50mg vials.

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ml
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Results

Concentration

10000 mcg/ml

10 mg/ml

Volume to inject

1 ml

per dose

Units on insulin syringe (U-100)

100 units

on a standard 100-unit insulin syringe

Doses per vial

5 doses

at 10000 mcg each

Large injection volume. Consider using more BAC water or a lower dose — volumes above 0.5ml can be uncomfortable to inject subcutaneously.

How this is calculated

50mg × 1,000 = 50000 mcg total

50000 ÷ 5ml = 10000 mcg/ml

10000 mcg ÷ 10000 = 1 ml

1 ml × 100 = 100 units

Disclaimer: This calculator is provided for informational purposes only. Always verify results against your specific vial labelling before use. Peptidy does not provide medical advice — consult a qualified healthcare professional before beginning or modifying any protocol. Use of research peptides may be subject to legal restrictions in your jurisdiction.

Dosing note. SS-31 doses are often quoted in milligrams (mg). This calculator uses micrograms (mcg) — multiply mg by 1,000. So 5mg = 5,000mcg and 10mg = 10,000mcg.

SS-31 is a mitochondria-targeted peptide researched for cellular energy and recovery, dosed by subcutaneous injection. Reported research doses vary, so calculate your concentration and units from your own vial rather than assuming a standard amount.

SS-31 dosing reference

Ranges reported in research use vary considerably. Clinical elamipretide studies have used higher subcutaneous doses; always calculate from your own vial and dose.

Lower daily dose5 mg (5,000 mcg) once daily
Higher daily dose10 mg (10,000 mcg) once daily

Worked example: 50mg vial + 5ml BAC water, 10mg dose

  • 50mg × 1,000 = 50,000 mcg total in the vial
  • 50,000 mcg ÷ 5ml = 10,000 mcg/ml concentration
  • 10,000 mcg (10mg) ÷ 10,000 mcg/ml = 1.0ml to inject
  • 1.0ml × 100 = 100 units on a U-100 insulin syringe
  • 50,000 mcg ÷ 10,000 mcg = 5 doses per vial

SS-31 reconstitution — frequently asked questions

How much BAC water do I add to a 50mg SS-31 vial?

5ml gives 10,000 mcg/ml, so a 10mg dose is exactly 1ml = 100 units. If you dose lower, a 10mg vial in 2ml gives 5,000 mcg/ml and a 5mg dose becomes 1ml = 100 units.

How many units is 10mg of SS-31?

With a 50mg vial reconstituted in 5ml (10,000 mcg/ml): 10,000 ÷ 10,000 = 1.0ml = 100 units. Enter your own vial size and water volume above to match your dose.

What is SS-31 also known as?

SS-31 is also referred to as elamipretide (and by the research code MTP-131). It is a mitochondria-targeted peptide; the reconstitution calculation is the same whichever name your vial uses.

How should reconstituted SS-31 be stored?

Refrigerate at 2–8°C away from light and label the vial with the date. Reconstituted SS-31 is commonly used within 2–4 weeks. Do not freeze it.

Track your SS-31 protocol in Peptidy

Store your vial concentration, log every dose against it, and never lose track of what you injected or when.